ARG64058
anti-XRCC4 like factor / NHEJ1 antibody
anti-XRCC4 like factor / NHEJ1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes XRCC4 like factor / NHEJ1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | XRCC4 like factor / NHEJ1 |
| Antigen Species | Human |
| Immunogen | C-QRPQLSKVKRKKPR |
| Conjugation | Un-conjugated |
| Alternate Names | XLF; Protein cernunnos; XRCC4-like factor; Non-homologous end-joining factor 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9H9Q4 Human Non-homologous end-joining factor 1 |
|---|---|
| Background | Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 33 kDa |
Images (2) Click the Picture to Zoom In
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ARG64058 anti-XRCC4 like factor / NHEJ1 antibody WB image
Western blot: Human Thyroid lysate (35 µg protein in RIPA buffer) stained with ARG64058 anti-XRCC4 like factor / NHEJ1 antibody at 0.1 µg/ml dilution.
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ARG64058 anti-XRCC4 like factor / NHEJ1 antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Placenta. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64058 anti-XRCC4 like factor / NHEJ1 antibody at 3.8 µg/ml dilution followed by AP-staining.
