ARG63711

anti-XAGE1 antibody

anti-XAGE1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description

Goat Polyclonal antibody recognizes XAGE1

Tested Reactivity Hu
Tested Application IHC-P
Specificity This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name XAGE1
Antigen Species Human
Immunogen CGFGFRRQGEDNT
Conjugation Un-conjugated
Alternate Names XAGE1C; XAGE1D; XAGE1; CTP9; Cancer/testis antigen 12.1; XAGE-1; G antigen family D member 2; X antigen family member 1; CT12.1D; CT12.1E; CT12.1C; GAGED2; CT12.1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 6 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 653067 Human XAGE1E

Swiss-port # Q9HD64 Human X antigen family member 1

Background This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene, in addition to alternative transcription start sites, results in multiple transcript variants. [provided by RefSeq, Jan 2010]
Research Area Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 9 kDa

Images (1) Click the Picture to Zoom In

  • ARG63711 anti-XAGE1 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Testis. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63711 anti-XAGE1 (Isoform 1d) / GAGED2 antibody at 3.8 µg/ml dilution followed by AP-staining.