ARG55017
anti-WDR82 antibody
anti-WDR82 antibody for ICC/IF,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes WDR82 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | WDR82 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 12-46 (N-terminus) of Human WDR82. |
| Conjugation | Un-conjugated |
| Alternate Names | PRO34047; Swd2; Protein TMEM113; WD repeat-containing protein 82; MST107; MSTP107; PRO2730; TMEM113; SWD2; WDR82A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | SH-SY5Y |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | WDR82 |
| Gene Full Name | WD repeat domain 82 |
| Background | TMEM113 (WDR82) is a component of the mammalian SET1A (MIM 611052)/SET1B (MIM 611055) histone H3-Lys4 methyltransferase complexes (Lee and Skalnik, 2005 [PubMed 16253997]; Lee et al., 2007 [PubMed 17355966]).[supplied by OMIM, Jul 2010] |
| Function | Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. [UniProt] |
| Cellular Localization | Nucleus Note=Associates with chromatin |
| Research Area | Gene Regulation antibody |
| Calculated MW | 35 kDa |
Images (2) Click the Picture to Zoom In
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ARG55017 anti-WDR82 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55017 anti-WDR82 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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ARG55017 anti-WDR82 antibody WB image
Western blot: 20 µg of SH-SY5Y cell lysate stained with ARG55017 anti-WDR82 antibody at 1:1000 dilution.
