ARG63840
anti-VPS11 antibody
anti-VPS11 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes VPS11 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VPS11 |
| Antigen Species | Human |
| Immunogen | C-HALQEKDTQTKLE |
| Conjugation | Un-conjugated |
| Alternate Names | END1; Vacuolar protein sorting-associated protein 11 homolog; hVPS11; RING finger protein 108; PEP5; RNF108 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9H270 Human Vacuolar protein sorting-associated protein 11 homolog |
|---|---|
| Background | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 108 kDa |
Images (2) Click the Picture to Zoom In
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ARG63840 anti-VPS11 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney-. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63840 anti-VPS11 antibody at 3.8 µg/ml dilution followed by AP-staining.
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ARG63840 anti-VPS11 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Pancreas. (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63840 anti-VPS11 antibody at 4 µg/ml dilution followed by HRP-staining.
