ARG42065
anti-VEGF antibody (Biotin)
anti-VEGF antibody (Biotin) for ELISA,Western blot and Human
Overview
| Product Description | Biotin-conjugated Goat Polyclonal antibody recognizes VEGF |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VEGF |
| Antigen Species | Human |
| Immunogen | Recombinant Human VEGF. |
| Conjugation | Biotin |
| Alternate Names | MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A |
Application Instructions
| Application Suggestion |
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| Application Note | Sandwich ELISA (Capture antibody - Detection antibody): ARG42064 (0.5 - 2 µg/ml) - ARG42065 (0.25 - 1 µg/ml) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P15692 Human Vascular endothelial growth factor A |
|---|---|
| Gene Symbol | VEGFA |
| Gene Full Name | vascular endothelial growth factor A |
| Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). [UniProt] |
| Cellular Localization | Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. [UniProt] |
| Highlight | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Anti-Goat IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| Calculated MW | 22 kDa |
Images (1) Click the Picture to Zoom In
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ARG42065 anti-VEGF antibody (Biotin) ELISA image
Sandwich ELISA: Detecting human VEGF (using 100 µl/well antibody solution). ARG42064 anti-VEGF antibody as a capture antibody at 0.5 - 2 µg/ml dilution in combination with ARG42065 anti-VEGF antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml dilution. Results of a typical standard run with optical density reading at 405 - 650 nm.
