ARG56630
anti-VEGF antibody
anti-VEGF antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes VEGF |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, IHC-P, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | VEGF |
| Antigen Species | Mouse |
| Immunogen | E.coli derived Recombinant Mouse VEGF. (MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR) |
| Conjugation | Un-conjugated |
| Alternate Names | MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q00731 Mouse Vascular endothelial growth factor A |
|---|---|
| Gene Symbol | Vegfa |
| Gene Full Name | vascular endothelial growth factor A |
| Background | This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008] |
| Function | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. [UniProt] |
| Highlight | Related products: VEGF antibodies; VEGF ELISA Kits; VEGF Duos / Panels; VEGF recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| Calculated MW | 27 kDa |
Images (6) Click the Picture to Zoom In
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ARG56630 anti-VEGF antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal murine kidney. The recommended ARG56630 anti-VEGF antibody concentration is 2.5 µg/ml for one hour at RT. A secondary fluorophore conjugated antibody was used for thirty minutes at RT. Antigen Retrieval: Boil tissue section in a buffer (High pH) followed by cooling at RT for 20 min.
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ARG56630 anti-VEGF antibody WB image
Western blot: 250 - 0.24 ng of Mouse VEGF stained with ARG56630 anti-VEGF antibody, under reducing conditions.
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ARG56630 anti-VEGF antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal murine kidney. The recommended ARG56630 anti-VEGF antibody concentration is 2.5 µg/ml for one hour at RT. A secondary fluorophore conjugated antibody was used for thirty minutes at RT. Antigen Retrieval: Boil tissue section in a buffer (High pH) followed by cooling at RT for 20 min.
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ARG56630 anti-VEGF antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal murine kidney. The recommended ARG56630 anti-VEGF antibody concentration is 2.5 µg/ml for one hour at RT. A secondary fluorophore conjugated antibody was used for thirty minutes at RT. Antigen Retrieval: Boil tissue section in a buffer (High pH) followed by cooling at RT for 20 min.
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ARG56630 anti-VEGF antibody WB image
Western blot: 250 - 0.24 ng of Mouse VEGF stained with ARG56630 anti-VEGF antibody, under non-reducing conditions.
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ARG56630 anti-VEGF antibody standard curve image
Sandwich ELISA: ARG56630 anti-VEGF antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56739 anti-VEGF antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
