ARG56963
anti-UBE2L6 antibody [k1H3]
anti-UBE2L6 antibody [k1H3] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [k1H3] recognizes UBE2L6 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | k1H3 |
| Isotype | IgG2b, kappa |
| Target Name | UBE2L6 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-152 of Human UBE2L6. |
| Conjugation | Un-conjugated |
| Alternate Names | UbcH8; EC 6.3.2.19; Ubiquitin-protein ligase L6; UBCH8; Ubiquitin/ISG15-conjugating enzyme E2 L6; RIG-B; Ubiquitin carrier protein L6; Retinoic acid-induced gene B protein |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O14933 Human Ubiquitin/ISG15-conjugating enzyme E2 L6 |
|---|---|
| Gene Symbol | UBE2L6 |
| Gene Full Name | ubiquitin-conjugating enzyme E2L 6 |
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011] |
| Function | Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3. [UniProt] |
| Calculated MW | 18 kDa |
| PTM | ISGylated. |
Images (5) Click the Picture to Zoom In
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ARG56963 anti-UBE2L6 antibody [k1H3] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56963 anti-UBE2L6 antibody [k1H3] IHC-P image
Immunohistochemistry: Paraffin embedded sections of Human breast lobule tissue stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:50 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
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ARG56963 anti-UBE2L6 antibody [k1H3] WB image
Western blot: 20 µg of MCF7 cell lysate stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:2000.
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ARG56963 anti-UBE2L6 antibody [k1H3] WB image
Western blot: 5 µg of 1) 293T cell lysate, 2) UBE2L6 Transfected 293T cell lysate stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:2000.
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ARG56963 anti-UBE2L6 antibody [k1H3] WB image
Western blot: 20 µg of Jurkat cell lysate stained with ARG56963 anti-UBE2L6 antibody [k1H3] at 1:2000.
