ARG65187
anti-Themis antibody
anti-Themis antibody for Western blot and Mouse
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Themis |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Themis |
| Antigen Species | Mouse |
| Immunogen | C-EERTINLPKSLKSH |
| Conjugation | Un-conjugated |
| Alternate Names | C6orf207; C6orf190; SPOT; Thymocyte-expressed molecule involved in selection; bA325O24.3; Protein THEMIS; bA325O24.4; GASP; TSEPA |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Themis |
| Gene Full Name | thymocyte selection associated |
| Background | This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009] |
| Function | Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. [UniProt] |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | 73 kDa |
| PTM | Phosphorylated on Tyr residues quickly after TCR stimulation. |
Images (1) Click the Picture to Zoom In
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ARG65187 anti-Themis antibody WB image
Western Blot: Mouse Spleen lysate (35 µg protein in RIPA buffer) stained with ARG65187 anti-Themis antibody at 0.3 µg/ml dilution.
