ARG42935
anti-TIMP2 antibody
anti-TIMP2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TIMP2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TIMP2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within aa. 100-200 of Human TIMP2 (NP_003246.1). |
| Conjugation | Un-conjugated |
| Alternate Names | TIMP-2; Tissue inhibitor of metalloproteinases 2; CSC-21K; DDC8; Metalloproteinase inhibitor 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | A549 | ||||||||
| Observed Size | ~ 26 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TIMP2 |
| Gene Full Name | TIMP metallopeptidase inhibitor 2 |
| Background | This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008] |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 24 kDa |
| PTM | The activity of TIMP2 is dependent on the presence of disulfide bonds. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG42935 anti-TIMP2 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG42935 anti-TIMP2 antibody at 1:100 dilution.
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ARG42935 anti-TIMP2 antibody WB image
Western blot: A549 cell lysate stained with ARG42935 anti-TIMP2 antibody at 1:1000 dilution.
