ARG43615
anti-TCP1 alpha antibody [2E7]
anti-TCP1 alpha antibody [2E7] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [2E7] recognizes TCP1 alpha |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | Detects ~60kDa. Also detects ~92kDa. Cross reactivity with Mouse HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2E7 |
| Isotype | IgG1 |
| Target Name | TCP1 alpha |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a.a. 515-551 within C-terminal region of Human TCP1 alpha. |
| Conjugation | Un-conjugated |
| Protein Full Name | T-complex protein 1 subunit alpha |
| Alternate Names | T-complex protein 1 subunit alpha; CCT1; D6S230E; TCP-1-alpha; CCTa; CCT-alpha |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TCP1 |
| Gene Full Name | t-complex protein 1 |
| Background | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded Monopeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010] |
| Function | Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. [UniProt] |
| Cellular Localization | Cytoplasm |
| Calculated MW | 60 kDa |
| PTM | Acetylation; Phosphoprotein |
Images (4) Click the Picture to Zoom In
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ARG43615 anti-TCP1 alpha antibody [2E7] IHC-P image
Immunohistochemistry: Human lung cancer stained with ARG43615 anti-TCP1 alpha antibody [2E7] at 2 μg/ml dilution.
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ARG43615 anti-TCP1 alpha antibody [2E7] ICC/IF image
Immunofluorescence: MCF7 stained with ARG43615 anti-TCP1 alpha antibody [2E7] at 2 μg/ml dilution.
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ARG43615 anti-TCP1 alpha antibody [2E7] WB image
Western blot: Hela, MCF-7, COLO-320 and HepG2 stained with ARG43615 anti-TCP1 alpha antibody [2E7] at 0.5 μg/mL dilution.
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ARG43615 anti-TCP1 alpha antibody [2E7] FACS image
Flow Cytometry: HepG2 stained with ARG43615 anti-TCP1 alpha antibody [2E7] at 1 μg / 10^6 cells dilution.
