ARG66600

anti-STAT2 phospho (Tyr690) antibody

anti-STAT2 phospho (Tyr690) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes STAT2 phospho (Tyr690)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Specificity The antibody detects endogenous levels of STAT2 protein only when phosphorylated at Tyr690.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name STAT2
Antigen Species Human
Immunogen Phosphospecific peptide corresponding to 656-705 of Human STAT2.
Conjugation Un-conjugated
Alternate Names P113; Signal transducer and activator of transcription 2; STAT113; p113; ISGF-3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:300
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20847 Mouse STAT2

GeneID: 288774 Rat STAT2

GeneID: 6773 Human STAT2

Swiss-port # P52630 Human Signal transducer and activator of transcription 2

Gene Symbol STAT2
Gene Full Name signal transducer and activator of transcription 2, 113kDa
Background The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
Function Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta. [UniProt]
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Calculated MW 98 kDa
PTM Tyrosine phosphorylated in response to IFN-alpha. Phosphorylation at Ser-287 negatively regulates the transcriptional response. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG66600 anti-STAT2 phospho (Tyr690) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue stained with ARG66600 anti-STAT2 phospho (Tyr690) antibody. The picture on the right is blocked with the phosphopeptide.

  • ARG66600 anti-STAT2 phospho (Tyr690) antibody WB image

    Western blot: HeLa cell lysate stained with ARG66600 anti-STAT2 phospho (Tyr690) antibody.