ARG42026
anti-SQSTM1 / p62 antibody [3H11]
anti-SQSTM1 / p62 antibody [3H11] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Autophagy Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [3H11] recognizes SQSTM1 / p62 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3H11 |
| Isotype | IgG2a |
| Target Name | SQSTM1 / p62 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 69-96 of Human SQSTM1 / p62. (DEDGDLVAFSSDEELTMAMSYVKDDIFR) |
| Conjugation | Un-conjugated |
| Alternate Names | FTDALS3; p60; p62; EBI3-associated protein of 60 kDa; p62B; Sequestosome-1; Ubiquitin-binding protein p62; PDB3; OSIL; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; EBIAP; A170; ZIP3 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 62 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SQSTM1 |
| Gene Full Name | sequestosome 1 |
| Background | This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009] |
| Function | Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. [UniProt] |
| Cellular Localization | Cytoplasm, cytosol. Late endosome. Lysosome. Cytoplasmic vesicle, autophagosome. Nucleus. Endoplasmic reticulum. Nucleus, PML body. Cytoplasm, myofibril, sarcomere. [UniProt] |
| Highlight | Related products: SQSTM1 antibodies; SQSTM1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Keap1-Nrf2-ARE antibody panel is launched Therapeutic strategies against PDAC |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Autophagy Study antibody |
| Calculated MW | 48 kDa |
| PTM | Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2 (PubMed:25040165). Ubiquitinated by RNF26: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed:27368102). Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery (PubMed:27368102). [UniProt] |
Images (10) Click the Picture to Zoom In
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] ICC/IF image
Immunofluorescence: A431 cells stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] WB image
Western blot: 50 µg of samples under reducing conditions. CCRF-CEM, HepG2, HT1080 and SW620 whole cell lysates stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 1 µg/ml dilution, overnight at 4°C.
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] WB image
Western blot: 50 µg of samples under reducing conditions. Rat brain, Rat liver, PC-12, RH35, Mouse brain, Mouse liver, NIH/3T3, Raw246.7 and Neuro-2a whole cell lysates stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42026 anti-SQSTM1 / p62 antibody [3H11] FACS image
Flow Cytometry: PC-3 cells were blocked with 10% normal goat serum and then stained with ARG42026 anti-SQSTM1 / p62 antibody [3H11] (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Mouse IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
