ARG63617
anti-SPRY1 / Sprouty 1 antibody
anti-SPRY1 / Sprouty 1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SPRY1 / Sprouty 1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | NP_005832.1 and NP_955359.1 represent varients of the same protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SPRY1 / Sprouty 1 |
| Antigen Species | Human |
| Immunogen | CPSRGQGKPS |
| Conjugation | Un-conjugated |
| Alternate Names | hSPRY1; Protein sprouty homolog 1; Spry-1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SPRY1 |
| Gene Full Name | sprouty homolog 1, antagonist of FGF signaling (Drosophila) |
| Function | May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 35 kDa |
Images (4) Click the Picture to Zoom In
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ARG63617 anti-SPRY1 / Sprouty 1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63617 anti-SPRY1 / Sprouty 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining.
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ARG63617 anti-SPRY1 / Sprouty 1 antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG63617 anti-SPRY1 / Sprouty 1 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG63617 anti-SPRY1 / Sprouty 1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63617 anti-SPRY1 / Sprouty 1 antibody at 10 µg/ml dilution for 1 hour (blue line) or Goat IgG for isotype control (black line), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
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ARG63617 anti-SPRY1 / Sprouty 1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63617 anti-SPRY1 / Sprouty 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining.
