ARG59535
anti-SMPD2 / nSMase antibody
anti-SMPD2 / nSMase antibody for Flow cytometry,Western blot and Human
Overview
Product Description | Rabbit Polyclonal antibody recognizes SMPD2 / nSMase |
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Tested Reactivity | Hu |
Tested Application | FACS, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | SMPD2 / nSMase |
Antigen Species | Human |
Immunogen | KLH-conjugated synthetic peptide between aa. 9-35 of Human SMPD2 / nSMase. |
Conjugation | Un-conjugated |
Alternate Names | ISC1; EC 3.1.4.12; NSMASE1; Lyso-platelet-activating factor-phospholipase C; nSMase; Lyso-PAF-PLC; Sphingomyelin phosphodiesterase 2; N-SMase; NSMASE; Neutral sphingomyelinase |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | K562 |
Properties
Form | Liquid |
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Purification | Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Buffer | PBS and 0.09% (W/V) Sodium azide. |
Preservative | 0.09% (W/V) Sodium azide. |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | SMPD2 |
Gene Full Name | sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) |
Background | This gene encodes a protein which was initially identified as a sphingomyelinase based on sequence similarity between bacterial sphingomyelinases and a yeast protein. Subsequent studies showed that its biological function is less likely to be as a sphingomyelinase and instead as a lysophospholipase. [provided by RefSeq, Oct 2009] |
Function | Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF. [UniProt] |
Cellular Localization | Membrane; Multi-pass membrane protein. [UniProt] |
Calculated MW | 48 kDa |
Images (2) Click the Picture to Zoom In
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ARG59535 anti-SMPD2 / nSMase antibody WB image
Western blot: 20 µg of K562 cell lysate stained with ARG59535 anti-SMPD2 / nSMase antibody at 1:1000 dilution.
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ARG59535 anti-SMPD2 / nSMase antibody FACS image
Flow Cytometry: NCI-H460 cells stained with ARG59535 anti-SMPD2 / nSMase antibody (bottom histogram) or without primary antibody as control (top histogram), followed by FITC-labelled secondary antibody.