ARG63942
anti-SMAD9 antibody
anti-SMAD9 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SMAD9 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is not expected to cross-react with SMAD1 and SMAD5. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SMAD9 |
| Antigen Species | Human |
| Immunogen | C-HSEPLMPHNATYPD |
| Conjugation | Un-conjugated |
| Alternate Names | Mothers against decapentaplegic homolog 9; MADH9; SMAD8A; Smad9; SMAD8B; Mothers against DPP homolog 9; MADH6; PPH2; Madh6; SMAD family member 9; SMAD 9; MAD homolog 9; SMAD8 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15198 Human Mothers against decapentaplegic homolog 9 |
|---|---|
| Background | The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010] |
| Research Area | Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 52 kDa |
| PTM | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. |
Images (2) Click the Picture to Zoom In
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ARG63942 anti-SMAD9 antibody WB image
Western Blot: Human Skin lysate (35 µg protein in RIPA buffer) stained with ARG63942 anti-SMAD9 antibody at 0.3 µg/ml dilution.
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ARG63942 anti-SMAD9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63942 anti-SMAD9 antibody at 5 µg/ml dilution followed by AP-staining.
