ARG57998

anti-SLC7A11 / xCT antibody

anti-SLC7A11 / xCT antibody for Immunoprecipitation,Western blot and Human,Mouse,Rat

Ferroptosis/Oxytosis Study antibody
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publication_link Publication9

Overview

Product Description Rabbit Polyclonal antibody recognizes SLC7A11 / xCT
Tested Reactivity Hu, Ms, Rat
Tested Application IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SLC7A11 / xCT
Antigen Species Human
Immunogen Synthetic peptide derived from Human SLC7A11 / xCT.
Conjugation Un-conjugated
Alternate Names xCT; CCBR1; Cystine/glutamate transporter; Solute carrier family 7 member 11; Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1

Application Instructions

Application Suggestion
Tested Application Dilution
IP1:50
WB1:500 - 1:2000
Application Note Note: Based on the reference PMID: 35322867 This antibody may also suitable for ICC/IF application in human samples.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 60 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23657 Human SLC7A11

GeneID: 26570 Mouse SLC7A11

Swiss-port # Q9UPY5 Human Cystine/glutamate transporter

Swiss-port # Q9WTR6 Mouse Cystine/glutamate transporter

Gene Symbol SLC7A11
Gene Full Name solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
Background This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death. [provided by RefSeq, Sep 2011]
Function Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30337 Ferroptosis / Oxytosis Antibody Panel
Related products:
SLC7A11 antibodies; SLC7A11 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Ferroptosis/Oxytosis Antibody Panel is launched
Therapeutic strategies against PDAC
Research Area Ferroptosis/Oxytosis Study antibody
Calculated MW 55 kDa

Images (3) Click the Picture to Zoom In

  • ARG57998 anti-SLC7A11 / xCT antibody WB image

    Western blot: Mouse Testis stained with ARG57998 anti-SLC7A11 / xCT antibody at 1:1000 dilution.

    From Lipeng Li et al. Ecotoxicol Environ Saf (2023), doi: 10.1016/j.ecoenv.2023.115309, Fig. 2F1.

  • ARG57998 anti-SLC7A11 / xCT antibody WB image

    Western blot: 20 μg of HeLa cell lysate stained with ARG57998 anti-SLC7A11 / xCT antibody at 1:1000 dilution.

  • ARG57998 anti-SLC7A11 / xCT antibody WB image

    Western blot: 20 μg of Mouse liver lysate stained with ARG57998 anti-SLC7A11 / xCT antibody at 1:1000 dilution.

Customer's Feedback

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Review for anti-SLC7A11 / xCT antibody

Application:WB

Sample:Mouse liver

Sample Loading Amount:20 μg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Inhibition of circRNA NGFR promotes ferroptosis in gallbladder carcinoma cells

WB / Human

Desen Fan et al.
Heliyon.,  (2024)

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Mechanism of YY1 mediating autophagy dependent ferroptosis in PM2.5 induced cardiac fibrosis

WB / Human

Huaifang Hu et al.
Chemosphere.,  (2023)

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FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice

WB / Mouse

Lipeng Li et al.
Ecotoxicol Environ Saf.,  (2023)

publication_link

 

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Liraglutide Alleviates Cognitive Deficit in db/db Mice: Involvement in Oxidative Stress, Iron Overload, and Ferroptosis

WB / Mouse

Ji-Ren An et al.
Neurochem Res.,  (2022)

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Blockage of Nrf2 and autophagy by L-selenocystine induces selective death in Nrf2-addicted colorectal cancer cells through p62-Keap-1-Nrf2 axis

WB / Human

Wei-Lun Hsu et al.
Cell Death Dis,  (2022)

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PM2.5 exposure triggers cell death through lysosomal membrane permeabilization and leads to ferroptosis insensitivity via the autophagy dysfunction/p62-KEAP1-NRF2 activation in neuronal cells

WB / Mouse

Min Wei et al.
Ecotoxicol Environ Saf,  (2022)

publication_link

 

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Fermented and Aged Ginseng Sprouts ( Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis

WB / Mouse

Ji Hyeon Ryu et al.
Antioxidants (Basel),  (2022)

publication_link

 

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IFNγ enhances ferroptosis by increasing JAK‑STAT pathway activation to suppress SLCA711 expression in adrenocortical carcinoma

WB, ICC/IF / Human

Xinbo Yu et al.
Oncol Rep.,  (2022)

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Oyster-Derived Tyr-Ala (YA) Peptide Prevents Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure by Suppressing Inflammatory, Apoptotic, Ferroptotic, and Pyroptotic Signals

WB / Mouse

Adrian S Siregar et al.
Mar Drugs.,  (2021)

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