ARG66123

anti-SF20 antibody (Biotin)

anti-SF20 antibody (Biotin) for ELISA,Western blot and Mouse

Overview

Product Description Biotin-conjugated Rabbit Polyclonal antibody recognizes SF20
Tested Reactivity Ms
Tested Application ELISA, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SF20
Antigen Species Mouse
Immunogen E. coli derived recombinant Mouse SF20.
(MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL)
Conjugation Biotin
Alternate Names EUROIMAGE1875335; IL27w; MYDGF; Interleukin-25; R33729_1; Myeloid-derived growth factor; IL-25; Stromal cell-derived growth factor SF20; IL25; IL27; SF20; C19orf10

Application Instructions

Application Suggestion
Tested Application Dilution
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66122 as a capture antibody
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 28106 Mouse MYDGF

Swiss-port # Q9CPT4 Mouse Myeloid-derived growth factor

Gene Symbol Mydgf
Gene Full Name myeloid derived growth factor
Background The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown. [provided by RefSeq, Jul 2008]
Function Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (By similarity). Involved in endothelial cell proliferation and angiogenesis. [UniProt]
Calculated MW 19 kDa

Images (4) Click the Picture to Zoom In

  • ARG66123 anti-SF20 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Mouse SF-20 stained with ARG66123 anti-SF20 antibody (Biotin), under non-reducing conditions.

  • ARG66123 anti-SF20 antibody (Biotin) standard curve image

    Direct ELISA: ARG66123 anti-SF20 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66123 anti-SF20 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Mouse SF-20 stained with ARG66123 anti-SF20 antibody (Biotin), under reducing conditions.

  • ARG66123 anti-SF20 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG66123 anti-SF20 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66122 anti-SF20 antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.