ARG63169
anti-SEC61A1 antibody
anti-SEC61A1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes SEC61A1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SEC61A1 |
| Antigen Species | Human |
| Immunogen | C-KEQSEVGSMGALLF |
| Conjugation | Un-conjugated |
| Alternate Names | Sec61 alpha-1; Protein transport protein Sec61 subunit alpha isoform 1; SEC61; HSEC61; SEC61A |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P61619 Human Protein transport protein Sec61 subunit alpha isoform 1 |
|---|---|
| Background | The protein encoded by this gene belongs to the SECY/SEC61- alpha family. It appears to play a crucial role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum. This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 52 kDa |
Images (2) Click the Picture to Zoom In
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ARG63169 anti-SEC61A1 antibody WB image
Western Blot: Human Brain (Cerebellum) lysate (35 µg protein in RIPA buffer) stained with ARG63169 anti-SEC61A1 antibody at 0.3 µg/ml dilution.
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ARG63169 anti-SEC61A1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63169 anti-SEC61A1 antibody at 3.75 µg/ml dilution followed by AP-staining.
