ARG54885

anti-SARS-CoV Spike protein antibody

anti-SARS-CoV Spike protein antibody for Western blot and Virus

Microbiology and Infectious Disease antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes SARS-CoV Spike protein.
Tested Reactivity Virus
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SARS-CoV Spike protein
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 532-562 of the middle of SARS-CoV Spike protein.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Function S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [UniProt]
Cellular Localization Virion membrane; Single-pass type I membrane protein. Host endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Note=Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly (By similarity). Some S oligomers are transported to the plasma membrane, where they may mediate cell- cell fusion.
Highlight Related products:
SARS-CoV antibodies; SARS-CoV ELISA Kits; SARS-CoV recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1, a biomarker and therapeutic target in COVID-19
ACE2, receptor of 2019-nCoV
Exploring Antiviral Immune Response
Research Area Microbiology and Infectious Disease antibody

Images (1) Click the Picture to Zoom In

  • ARG54885 anti-SARS-CoV Spike protein antibody WB image

    Western blot: Recombinant Spike proteins (aa. 17-537 or aa. 17-756) stained with ARG54885 anti-SARS-CoV Spike protein antibody.

Specific References

Assessing the application of a pseudovirus system for emerging SARS-CoV-2 and re-emerging avian influenza virus H5 subtypes in vaccine development.

WB / Virus

Sheng-Wen Huang et al.
Biomed J.,  (2020)

publication_link

 

hr_line