ARG62619
anti-Rhodopsin antibody [RET-P1]
anti-Rhodopsin antibody [RET-P1] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [RET-P1] recognizes Rhodopsin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | RET-P1 |
| Isotype | IgG1 |
| Target Name | Rhodopsin |
| Antigen Species | Rat |
| Immunogen | Membrane preparation from adult rat retina. |
| Conjugation | Un-conjugated |
| Alternate Names | Rhodopsin; Opsin-2; CSNBAD1; RP4; OPN2 |
Application Instructions
| Application Note | FACS: 1µg for 106 cells WB: 1 - 2 µg/ml IHC-P: 1/2 - 1/4 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|---|
| Positive Control | IMR-5 cells, brain or retina tissue sections. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Rho |
| Gene Full Name | rhodopsin |
| Background | Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008] |
| Function | Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity). [UniProt] |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 39 kDa |
| PTM | Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region. Contains one covalently linked retinal chromophore. |
Clone References
Transplantation of human bone marrow mesenchymal stem cells as a thin subretinal layer ameliorates retinal degeneration in a rat model of retinal dystrophy.
IHC-P /
Conditional ablation of the choroideremia gene causes age-related changes in mouse retinal pigment epithelium.
ICC/IF / Mouse
Leukemia inhibitory factor coordinates the down-regulation of the visual cycle in the retina and retinal-pigmented epithelium.
WB / Mouse
Spatiotemporal expression pattern of ceramide kinase-like in the mouse retina.
IHC-P / Mouse
Immunocytochemical study of rhodopsin-containing putative encephalic photoreceptors in house sparrow, Passer domesticus.
IHC / House Sparrow
IMP dehydrogenase type 1 associates with polyribosomes translating rhodopsin mRNA.
IP / Cow
Characterization of a canine model of autosomal recessive retinitis pigmentosa due to a PDE6A mutation.
IHC / Dog
Conversion of mammalian Müller glial cells into a neuronal lineage by in vitro aggregate-culture.
IHC / Rat
