ARG45311
anti-RTF1 antibody
anti-RTF1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Monkey
Overview
| Product Description | Polyclonal antibody recognizes RTF1 |
|---|---|
| Tested Reactivity | Hu, Mk |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RTF1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human RTF1. |
| Conjugation | Un-conjugated |
| Alternate Names | RTF1; RTF1 Homolog, Paf1/RNA Polymerase II Complex Component; KIAA0252; RNA Polymerase-Associated Protein RTF1 Homolog; Rtf1, Paf1/RNA Polymerase II Complex Component, Homolog (S. Cerevisiae); Rtf1, Paf1/RNA Polymerase II Complex Component, Homolog; Ortholog Of Mouse Gene Trap Locus 7; GTL7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 100 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q92541 Human RNA polymerase-associated protein RTF1 homolog |
|---|---|
| Gene Symbol | RTF1 |
| Gene Full Name | RTF1 Homolog, Paf1/RNA Polymerase II Complex Component |
| Background | This locus may represent a gene involved in regulation of transcription elongation and chromatin remodeling, based on studies of similar proteins in other organisms. The encoded protein may bind single-stranded DNA. [provided by RefSeq, Sep 2010] |
| Function | Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex . [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 80 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG45311 anti-RTF1 antibody WB image
Western blot: SW620 and MCF-7 stained with ARG45311 anti-RTF1 antibody at 0.5 μg/ml dilution.
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ARG45311 anti-RTF1 antibody FACS image
Flow Cytometry: Daudi stained with ARG45311 anti-RTF1 antibody at 1 µg/10^6 cells dilution.
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ARG45311 anti-RTF1 antibody IHC-P image
Immunohistochemistry: Rat brain stained with ARG45311 anti-RTF1 antibody at 2 μg/ml dilution.
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ARG45311 anti-RTF1 antibody IHC-P image
Immunohistochemistry: Mouse brain stained with ARG45311 anti-RTF1 antibody at 2 μg/ml dilution.
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ARG45311 anti-RTF1 antibody WB image
Western blot: COS-7 stained with ARG45311 anti-RTF1 antibody at 0.5 μg/ml dilution.
