ARG45307

anti-RPS15A antibody

anti-RPS15A antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Polyclonal antibody recognizes RPS15A
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RPS15A
Antigen Species Human
Immunogen Recombinant protein containing to human RPS15A.
Conjugation Un-conjugated
Alternate Names RPS15A; Ribosomal Protein S15a; S15A; US8; Small Ribosomal Subunit Protein US8; 40S Ribosomal Protein S15a; Up-Regulated By HBV X Protein; DBA20

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.1-0.25 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 15 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117053 Rat RPS15A

GeneID: 267019 Mouse RPS15A

GeneID: 6210 Human RPS15A

Gene Symbol RPS15A
Gene Full Name Ribosomal Protein S15a
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Function Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome . [UniProt]
Cellular Localization Cytoplasm; Nucleus. [UniProt]
Calculated MW 15 kDa

Images (6) Click the Picture to Zoom In

  • ARG45307 anti-RPS15A antibody IHC-P image

    Immunohistochemistry: Human colon adenocarcinoma stained with ARG45307 anti-RPS15A antibody at 2 μg/ml dilution.

  • ARG45307 anti-RPS15A antibody ICC/IF image

    Immunofluorescence: Hela stained with ARG45307 anti-RPS15A antibody at 5 ug/ml dilution.

  • ARG45307 anti-RPS15A antibody WB image

    Western blot: HeoG2 and THP-1 stained with ARG45307 anti-RPS15A antibody at 0.5 μg/ml dilution.

  • ARG45307 anti-RPS15A antibody FACS image

    Flow Cytometry: HL-60 stained with ARG45307 anti-RPS15A antibody at 1 µg/10^6 cells dilution.

  • ARG45307 anti-RPS15A antibody WB image

    Western blot: RH35 stained with ARG45307 anti-RPS15A antibody at 0.5 μg/ml dilution.

  • ARG45307 anti-RPS15A antibody WB image

    Western blot: NIH/3T3 stained with ARG45307 anti-RPS15A antibody at 0.5 μg/ml dilution.