ARG42223
anti-RMI2 antibody
anti-RMI2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RMI2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RMI2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence of Human RMI2. (KMTDLSDNPIHESMWELEVEDLHRNIP) |
| Conjugation | Un-conjugated |
| Alternate Names | C16orf75; BLM-associated protein of 18 kDa; BLAP18; RecQ-mediated genome instability protein 2; hRMI2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 19 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q3UPE3 Mouse RecQ-mediated genome instability protein 2 Swiss-port # Q96E14 Human RecQ-mediated genome instability protein 2 |
|---|---|
| Gene Symbol | RMI2 |
| Gene Full Name | RecQ mediated genome instability 2 |
| Background | RMI2 is a component of the BLM (RECQL3; MIM 604610) complex, which plays a role in homologous recombination-dependent DNA repair and is essential for genome stability (Xu et al., 2008 [PubMed 18923082]).[supplied by OMIM, Nov 2008] |
| Function | Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. Essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM. [UniProt] |
| Cellular Localization | Nucleus. Note=Colocalizes with BLM at nuclear DNA repair foci. [UniProt] |
| Calculated MW | 16 kDa |
| PTM | Phosphorylated during mitosis. [UniProt] |
Images (8) Click the Picture to Zoom In
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ARG42223 anti-RMI2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42223 anti-RMI2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42223 anti-RMI2 antibody WB image
Western blot: 50 µg of samples under reducing conditions. HeLa, T-47D, HepG2, K562, Rat thymus and Mouse HEPA1-6 cell lysates stained with ARG42223 anti-RMI2 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42223 anti-RMI2 antibody FACS image
Flow Cytometry: U2OS cells were blocked with 10% normal goat serum and then stained with ARG42223 anti-RMI2 antibody (blue line) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green line) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red line) was also used as a control.
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ARG42223 anti-RMI2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42223 anti-RMI2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42223 anti-RMI2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42223 anti-RMI2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42223 anti-RMI2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42223 anti-RMI2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42223 anti-RMI2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42223 anti-RMI2 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42223 anti-RMI2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse liver tissues. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42223 anti-RMI2 antibody at 1 µg/ml dilution, overnight at 4°C.
