ARG55861
anti-RBX1 / ROC1 antibody
anti-RBX1 / ROC1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RBX1 / ROC1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RBX1 / ROC1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 74-108 of Human RBX1 / ROC1. |
| Conjugation | Un-conjugated |
| Alternate Names | E3 ubiquitin-protein ligase RBX1; RING finger protein 75; EC 6.3.2.-; RNF75; Regulator of cullins 1; RING-box protein 1; ROC1; Protein ZYP; Rbx1; BA554C12.1 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RBX1 |
| Gene Full Name | ring-box 1, E3 ubiquitin protein ligase |
| Background | This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010] |
| Function | E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus |
| Highlight | Related products: RBX1 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Keap1-Nrf2-ARE antibody panel is launched |
| Calculated MW | 12 kDa |
Images (3) Click the Picture to Zoom In
-
ARG55861 anti-RBX1 / ROC1 antibody WB image
Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG55861 anti-RBX1 / ROC1 antibody at 1:1000 dilution.
-
ARG55861 anti-RBX1 / ROC1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55861 anti-RBX1 / ROC1 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
-
ARG55861 anti-RBX1 / ROC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis tissue stained with ARG55861 anti-RBX1 / ROC1 antibody at 1:100 dilution.
Customer's Feedback
Excellent
Review for anti-RBX1 / ROC1 antibody
Application：WB
Sample：HeLa and 293T
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
