ARG41284

anti-RASGRF1 / CDC25 antibody

anti-RASGRF1 / CDC25 antibody for Western blot and Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes RASGRF1 / CDC25
Tested Reactivity Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name RASGRF1 / CDC25
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-190 of Human RASGRF1 / CDC25 (NP_001139120.1).
Conjugation Un-conjugated
Alternate Names CDC25L; Ras-specific guanine nucleotide-releasing factor 1; CDC25; GRF1; PP13187; GRF55; H-GRF55; Ras-specific nucleotide exchange factor CDC25; Guanine nucleotide-releasing protein; Ras-GRF1; GNRP; ras-GRF1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse brain
Observed Size 145 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19417 Mouse RASGRF1

Swiss-port # P27671 Mouse Ras-specific guanine nucleotide-releasing factor 1

Gene Symbol RASGRF1
Gene Full Name Ras protein-specific guanine nucleotide-releasing factor 1
Background The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]
Function Promotes the exchange of Ras-bound GDP by GTP. [UniProt]
Calculated MW 145 kDa
PTM Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.

Ubiquitinated and degraded following phosphorylation by PLK2.

Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG41284 anti-RASGRF1 / CDC25 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human stomach tissue stained with ARG41284 anti-RASGRF1 / CDC25 antibody at 1:100 dilution.

  • ARG41284 anti-RASGRF1 / CDC25 antibody WB image

    Western blot: 25 ug of Mouse brain lysate stained with ARG41284 anti-RASGRF1 / CDC25 antibody at 1:1000 dilution.