ARG64568
anti-PRUNE2 / BMCC1 antibody
anti-PRUNE2 / BMCC1 antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Cell Death antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PRUNE2 / BMCC1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PRUNE2 / BMCC1 |
| Antigen Species | Human |
| Immunogen | C-MESEKISEKQEE |
| Conjugation | Un-conjugated |
| Alternate Names | KIAA0367; Protein prune homolog 2; C9orf65; BNIPXL; BMCC1; BNIP2 motif-containing molecule at the C-terminal region 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRUNE2 |
| Gene Full Name | prune homolog 2 (Drosophila) |
| Background | The protein encoded by this gene belongs to the B-cell CLL/lymphoma 2 and adenovirus E1B 19 kDa interacting family, whose members play roles in many cellular processes including apotosis, cell transformation, and synaptic function. Several functions for this protein have been demonstrated including suppression of Ras homolog family member A activity, which results in reduced stress fiber formation and suppression of oncogenic cellular transformation. A high molecular weight isoform of this protein has also been shown to colocalize with Adaptor protein complex 2, beta-Adaptin and endodermal markers, suggesting an involvement in post-endocytic trafficking. In prostate cancer cells, this gene acts as a tumor suppressor and its expression is regulated by prostate cancer antigen 3, a non-protein coding gene on the opposite DNA strand in an intron of this gene. Prostate cancer antigen 3 regulates levels of this gene through formation of a double-stranded RNA that undergoes adenosine deaminase actin on RNA-dependent adenosine-to-inosine RNA editing. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015] |
| Function | May play an important role in regulating differentiation, survival and aggressiveness of the tumor cells. [UniProt] |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody |
| Calculated MW | 341 kDa |
Images (1) Click the Picture to Zoom In
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ARG64568 anti-PRUNE2 / BMCC1 antibody WB image
Western Blot: Human Brain lysate (35 µg protein in RIPA buffer) stained with ARG64568 anti-PRUNE2 / BMCC1 antibody at 1 µg/ml dilution.
