ARG43977

anti-PRPF6 antibody

anti-PRPF6 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PRPF6
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PRPF6
Antigen Species Human
Immunogen Human PRPF6 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name Pre-mRNA-processing factor 6
Alternate Names PRPF6; Pre-MRNA Processing Factor 6; ANT-1; SNRNP102; C20orf14; U5-102K; HPrp6; Prp6; RP60; TOM; U5 SnRNP-Associated 102 KDa Protein; Pre-MRNA-Processing Factor 6; U5-102 KDa Protein; PRP6 Homolog; BB152O15.1; P102 U5 Small Nuclear Ribonucleoprotein Particle-Binding Protein ; Androgen Receptor N-Terminal Domain Transactivating Protein-1; Putative Mitochondrial Outer Membrane Protein Import Receptor; Androgen Receptor N-Terminal Domain-Transactivating Protein; PRP6 Pre-MRNA Processing Factor 6 Homolog; Chromosome 20 Open Reading Frame 14; ANT1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified with Immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24148 Human PRPF6

GeneID: 366276 Rat PRPF6

GeneID: 68879 Mouse PRPF6

Gene Symbol PRPF6
Gene Full Name Pre-MRNA Processing Factor 6
Background The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.
Function Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome.
Cellular Localization Nucleus, Spliceosome
Calculated MW 107 kDa
PTM Phosphoprotein

Images (5) Click the Picture to Zoom In

  • ARG43977 anti-PRPF6 antibody WB image ICC/IF image

    Immunofluorescence: A549 cells stained with ARG43977 anti-PRPF6 antibody WB image at 5 μg/ml dilution.

  • ARG43977 anti-PRPF6 antibody WB image

    Western blot: Hela and K562 stained with ARG43977 anti-PRPF6 antibody at 0.5 μg/mL dilution.

  • ARG43977 anti-PRPF6 antibody FACS image

    Flow Cytometry: Hela cells stained with ARG43977 anti-PRPF6 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43977 anti-PRPF6 antibody WB image

    Western blot: C6 cells stained with ARG43977 anti-PRPF6 antibody at 0.5 μg/mL dilution.

  • ARG43977 anti-PRPF6 antibody WB image

    Western blot: NIH/3T3 cells stained with ARG43977 anti-PRPF6 antibody at 0.5 μg/mL dilution.