ARG43977

anti-PRPF6 antibody

anti-PRPF6 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PRPF6
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Target Name PRPF6
Antigen Species Human
Immunogen Human PRPF6 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name Pre-mRNA-processing factor 6
Alternate Names PRPF6; Pre-MRNA Processing Factor 6; ANT-1; SNRNP102; C20orf14; U5-102K; HPrp6; Prp6; RP60; TOM; U5 SnRNP-Associated 102 KDa Protein; Pre-MRNA-Processing Factor 6; U5-102 KDa Protein; PRP6 Homolog; BB152O15.1; P102 U5 Small Nuclear Ribonucleoprotein Particle-Binding Protein ; Androgen Receptor N-Terminal Domain Transactivating Protein-1; Putative Mitochondrial Outer Membrane Protein Import Receptor; Androgen Receptor N-Terminal Domain-Transactivating Protein; PRP6 Pre-MRNA Processing Factor 6 Homolog; Chromosome 20 Open Reading Frame 14; ANT1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 24148 Human PRPF6

GeneID: 366276 Rat PRPF6

GeneID: 68879 Mouse PRPF6

Gene Symbol PRPF6
Gene Full Name Pre-MRNA Processing Factor 6
Background The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.
Function Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome.
Cellular Localization Nucleus, Spliceosome
Calculated MW 107 kDa
PTM Phosphoprotein

Images (5) Click the Picture to Zoom In

  • ARG43977 anti-PRPF6 antibody WB image ICC/IF image

    Immunofluorescence: A549 cells stained with ARG43977 anti-PRPF6 antibody WB image at 5 μg/ml dilution.

  • ARG43977 anti-PRPF6 antibody WB image

    Western blot: Hela and K562 stained with ARG43977 anti-PRPF6 antibody at 0.5 μg/mL dilution.

  • ARG43977 anti-PRPF6 antibody FACS image

    Flow Cytometry: Hela cells stained with ARG43977 anti-PRPF6 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43977 anti-PRPF6 antibody WB image

    Western blot: C6 cells stained with ARG43977 anti-PRPF6 antibody at 0.5 μg/mL dilution.

  • ARG43977 anti-PRPF6 antibody WB image

    Western blot: NIH/3T3 cells stained with ARG43977 anti-PRPF6 antibody at 0.5 μg/mL dilution.