ARG59949
anti-POLR2H / RPB8 antibody
anti-POLR2H / RPB8 antibody for Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes POLR2H / RPB8 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | POLR2H / RPB8 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-150 of Human POLR2H (NP_006223.2). |
| Conjugation | Un-conjugated |
| Alternate Names | DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; DNA-directed RNA polymerases I, II, and III subunit RPABC3; hRPB8; RPB8; RPB8 homolog; RPB17; RPABC3; DNA-directed RNA polymerase II subunit H; RNA polymerases I, II, and III subunit ABC3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse thymus and 293T | ||||||
| Observed Size | 17 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P52434 Human DNA-directed RNA polymerases I, II, and III subunit RPABC3 Swiss-port # Q923G2 Mouse DNA-directed RNA polymerases I, II, and III subunit RPABC3 |
|---|---|
| Gene Symbol | POLR2H |
| Gene Full Name | polymerase (RNA) II (DNA directed) polypeptide H |
| Background | The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. [UniProt] |
| Cellular Localization | Nucleus, nucleolus. [UniProt] |
| Calculated MW | 17 kDa |
Images (3) Click the Picture to Zoom In
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ARG59949 anti-POLR2H / RPB8 antibody WB image
Western blot: 25 µg of Mouse thymus and 293T cell lysates stained with ARG59949 anti-POLR2H / RPB8 antibody at 1:1000 dilution.
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ARG59949 anti-POLR2H / RPB8 antibody IP image
Immunoprecipitation: 200 µg extracts of MCF7 cells immunoprecipitated and stained with ARG59949 anti-POLR2H / RPB8 antibody at 1:1000 dilution.
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ARG59949 anti-POLR2H / RPB8 antibody WB image
Western blot: 25 µg of Mouse thymus and 293T cell lysates stained with ARG59949 anti-POLR2H / RPB8 antibody at 1:1000 dilution through one-step method.
