ARG44217
anti-POLR2B antibody
anti-POLR2B antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes POLR2B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | POLR2B |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human POLR2B |
| Conjugation | Un-conjugated |
| Protein Full Name | DNA-directed RNA polymerase II subunit RPB2 |
| Alternate Names | POLR2B; RNA Polymerase II Subunit B; RPB2; DNA-Directed RNA Polymerase II 140 KDa Polypeptide; DNA-Directed RNA Polymerase II Subunit RPB2; DNA-Directed RNA Polymerase II Subunit B; RNA Polymerase II Subunit B2; RNA Polymerase II Subunit 2; EC 2.7.7.6; Polymerase (RNA) II (DNA Directed) Polypeptide B (140kD); Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa; RNA Polymerase II Second Largest Subunit; Polymerase (RNA) II Subunit B; EC 2.7.7; HRPB140; POL2RB |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P30876 Human DNA-directed RNA polymerase II subunit RPB2 Swiss-port # Q8CFI7 Mouse DNA-directed RNA polymerase II subunit RPB2 |
|---|---|
| Gene Symbol | POLR2B |
| Gene Full Name | Rna Polymerase Ii Subunit B |
| Background | This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. |
| Cellular Localization | DNA-directed RNA polymerase, Nucleus |
| Calculated MW | 134 kDa |
| PTM | Methylation, Phosphoprotein |
Images (5) Click the Picture to Zoom In
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ARG44217 anti-POLR2B antibody ICC/IF image
Immunofluorescence: A549 stained with ARG44217 anti-POLR2B antibody at 5 μg/mL dilution.
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ARG44217 anti-POLR2B antibody WB image
Western blot: A431, HeLa, Ramos and U251 stained with ARG44217 anti-POLR2B antibody at 0.5 μg/mL dilution.
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ARG44217 anti-POLR2B antibody FACS image
Flow Cytometry: U251 stained with ARG44217 anti-POLR2B antibody at 1 μg/1x10^6 cells dilution.
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ARG44217 anti-POLR2B antibody WB image
Western blot: Rat brain and RH35 stained with ARG44217 anti-POLR2B antibody at 0.5 μg/mL dilution.
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ARG44217 anti-POLR2B antibody WB image
Western blot: Mouse brain and NIH/3T3 stained with ARG44217 anti-POLR2B antibody at 0.5 μg/mL dilution.
