ARG56710

anti-PLGF1 antibody

anti-PLGF1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes PLGF1
Tested Reactivity Hu
Tested Application ELISA, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PLGF1
Antigen Species Human
Immunogen E.coli derived Recombinant Human PlGF-1.
(MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR)
Conjugation Un-conjugated
Alternate Names PGFL; PlGF; PlGF-2; SHGC-10760; Placenta growth factor; D12S1900; PLGF

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56819 as a detection antibody
IHC-P2.0 µg/ml
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5228 Human PGF

Swiss-port # P49763 Human Placenta growth factor

Gene Symbol PGF
Gene Full Name placental growth factor
Background This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]
Function Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth. [UniProt]
Highlight Related products:
PLGF1 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
The role of HDGF in tumor angiogenesis
Calculated MW 25 kDa
PTM N-glycosylated.

Images (5) Click the Picture to Zoom In

  • ARG56710 anti-PLGF1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56710 anti-PLGF1 antibody concentration is 2.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56710 anti-PLGF1 antibody WB image

    Western blot: 250 - 0.24 ng of Human PlGF stained with ARG56710 anti-PLGF1 antibody, under reducing conditions.

  • ARG56710 anti-PLGF1 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56710 anti-PLGF1 antibody concentration is 2.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56710 anti-PLGF1 antibody WB image

    Western blot: 250 - 0.24 ng of Human PlGF stained with ARG56710 anti-PLGF1 antibody, under non-reducing conditions.

  • ARG56710 anti-PLGF1 antibody standard curve image

    Sandwich ELISA: ARG56710 anti-PLGF1 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56819 anti-PLGF1 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.