ARG63642
anti-PITPNM / PITPNM1 antibody
anti-PITPNM / PITPNM1 antibody for Flow cytometry,ICC/IF and Human
Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PITPNM / PITPNM1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Cow, Dog |
| Tested Application | FACS, ICC/IF |
| Specificity | This antibody is expected to recognise both reported isoforms. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PITPNM / PITPNM1 |
| Antigen Species | Human |
| Immunogen | C-KARSISLKLDSEE |
| Conjugation | Un-conjugated |
| Alternate Names | RDGBA1; Phosphatidylinositol transfer protein, membrane-associated 1; RDGB1; Membrane-associated phosphatidylinositol transfer protein 1; NIR2; Rd9; PITPNM; NIR-2; RDGB; DRES9; RDGBA; Pyk2 N-terminal domain-interacting receptor 2; Drosophila retinal degeneration B homolog; PITPnm 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00562 Human Membrane-associated phosphatidylinositol transfer protein 1 |
|---|---|
| Background | PITPNM1 belongs to a family of membrane-associated phosphatidylinositol transfer domain-containing proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein (Ocaka et al., 2005 [PubMed 15627748]).[supplied by OMIM, Mar 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 135 kDa |
| PTM | Phosphorylated on multiple sites by CDK1 at the onset of mitosis. Phosphorylation facilitates dissociation from the Golgi complex and is required for interaction with PLK1. Phosphorylated on threonine residues upon treatment with oleic acid. Phosphorylated on tyrosine residues by PTK2B. |
Images (2) Click the Picture to Zoom In
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ARG63642 anti-PITPNM / PITPNM1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A549 cells permeabilized with 0.15% Triton. Cells were stained with ARG63642 anti-PITPNM / PITPNM1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized Goat IgG at 10 µg/ml dilution.
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ARG63642 anti-PITPNM / PITPNM1 antibody FACS image
Flow Cytometry: Paraformaldehyde fixed A549 cells permeabilized with 0.5% Triton. Cells were stained with ARG63642 anti-PITPNM / PITPNM1 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa Fluor® 488 labelled secondary antibody. IgG control: Unimmunized Goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
