ARG22292
anti-PDI antibody
anti-PDI antibody for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Dog,Guinea pig,Hamster,Monkey,Pig,Sheep,Xenopus laevis
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PDI |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Dog, Gpig, Hm, Mk, Pig, Sheep, Xenopus laevis |
| Tested Application | ICC/IF, IHC, IP, WB |
| Specificity | Detects ~58kDa. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | PDI |
| Antigen Species | Rat |
| Immunogen | KLH-conjugated synthetic peptide from Rat PDI |
| Conjugation | Un-conjugated |
| Alternate Names | PDA2; Pancreas-specific protein disulfide isomerase; PDIR; PDIP; PDI; Protein disulfide-isomerase A2; PDIp; EC 5.3.4.1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Pdia2 |
| Gene Full Name | protein disulfide isomerase family A, member 2 |
| Background | Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions (Desilva et al., 1996 [PubMed 8561901]).[supplied by OMIM, Mar 2008] |
| Function | Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins. [UniProt] |
| Cellular Localization | Endoplasmic Reticulum, Endoplasmic reticulum lumen |
| Calculated MW | 58 kDa |
| PTM | The disulfide-linked homodimer exhibits an enhanced chaperone activity. Glycosylated. |
Images (3) Click the Picture to Zoom In
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ARG22292 anti-PDI antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed HeLa cells stained with ARG22292 anti-PDI antibody (yellow) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22292 anti-PDI antibody WB image
Western blot: 50 ng of PDI purified protein and 15 µg of Rat tissue/cell lysates stained with ARG22292 anti-PDI antibody at 1:4000 dilution.
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ARG22292 anti-PDI antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed HeLa cells stained with ARG22292 anti-PDI antibody (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
