ARG56814
anti-PDGF BB antibody (Biotin)
anti-PDGF BB antibody (Biotin) for ELISA,Western blot and Human
Overview
| Product Description | Biotin-conjugated Rabbit Polyclonal antibody recognizes PDGF BB |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDGF BB |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human PDGF-BB. (SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT) |
| Conjugation | Biotin |
| Alternate Names | SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01127 Human Platelet-derived growth factor subunit B |
|---|---|
| Gene Symbol | PDGFB |
| Gene Full Name | platelet-derived growth factor beta polypeptide |
| Background | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013] |
| Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity). [UniProt] |
| Highlight | Related products: PDGF BB antibodies; PDGF BB ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| Calculated MW | 27 kDa |
Images (4) Click the Picture to Zoom In
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ARG56814 anti-PDGF BB antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human PDGF-BB stained with ARG56814 anti-PDGF BB antibody (Biotin), under reducing conditions.
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ARG56814 anti-PDGF BB antibody (Biotin) standard curve image
Direct ELISA: ARG56814 anti-PDGF BB antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.
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ARG56814 anti-PDGF BB antibody (Biotin) WB image
Western blot: 250 - 0.24 ng of Human PDGF-BB stained with ARG56814 anti-PDGF BB antibody (Biotin), under non-reducing conditions.
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ARG56814 anti-PDGF BB antibody (Biotin) standard curve image
Sandwich ELISA: ARG56814 anti-PDGF BB antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56705 anti-PDGF-BB antibody as a capture antibody. Results of a typical standard run with optical density.
