ARG56705
anti-PDGF BB antibody
anti-PDGF BB antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PDGF BB |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDGF BB |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human PDGF-BB. (SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT) |
| Conjugation | Un-conjugated |
| Alternate Names | SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P01127 Human Platelet-derived growth factor subunit B |
|---|---|
| Gene Symbol | PDGFB |
| Gene Full Name | platelet-derived growth factor beta polypeptide |
| Background | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013] |
| Function | Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity). [UniProt] |
| Highlight | Related products: PDGF BB antibodies; PDGF BB ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| Calculated MW | 27 kDa |
Images (5) Click the Picture to Zoom In
-
ARG56705 anti-PDGF BB antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal Human skin. The recommended ARG56705 anti-PDGF BB antibody concentration is 1.0 µg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.
-
ARG56705 anti-PDGF BB antibody WB image
Western blot: 250 - 0.24 ng of Human PDGF-BB stained with ARG56705 anti-PDGF BB antibody, under reducing conditions.
-
ARG56705 anti-PDGF BB antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal Human skin. The recommended ARG56705 anti-PDGF BB antibody concentration is 1.0 µg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.
-
ARG56705 anti-PDGF BB antibody WB image
Western blot: 250 - 0.24 ng of Human PDGF-BB stained with ARG56705 anti-PDGF BB antibody, under non-reducing conditions.
-
ARG56705 anti-PDGF BB antibody standard curve image
Sandwich ELISA: ARG56705 anti-PDGF BB antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56814 anti-PDGF-BB antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
