ARG63488
anti-PAX8 antibody
anti-PAX8 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PAX8 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1) |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PAX8 |
| Antigen Species | Human |
| Immunogen | PHNSIRSGHGGLN-C |
| Conjugation | Un-conjugated |
| Alternate Names | Paired box protein Pax-8 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010] |
| Research Area | Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 48 kDa |
Images (4) Click the Picture to Zoom In
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ARG63488 anti-PAX8 antibody WB image
Western Blot: Human Kidney lysate (35 µg protein in RIPA buffer) stained with ARG63488 anti-PAX8 antibody at 1.5 µg/ml dilution.
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ARG63488 anti-PAX8 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63488 anti-PAX8 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63488 anti-PAX8 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Human Thyroid Gland. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63488 anti-PAX8 antibody at 2 µg/ml dilution followed by HRP-staining.
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ARG63488 anti-PAX8 antibody WB image
Western blot: 35 µg of Human kidney (A) and HeLa cell (B) lysates (in RIPA buffer) stained with ARG63488 anti-PAX8 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
Customer's Feedback
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Review for anti-PAX8 antibody
Application：WB
Sample：A498
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：6 µl in 2 ml
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
