ARG63434
anti-P114-RHO-GEF / ARHGEF18 antibody
anti-P114-RHO-GEF / ARHGEF18 antibody for Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes P114-RHO-GEF / ARHGEF18 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | P114-RHO-GEF / ARHGEF18 |
| Antigen Species | Human |
| Immunogen | C-SAKEDASKEDVIFF |
| Conjugation | Un-conjugated |
| Alternate Names | 114 kDa Rho-specific guanine nucleotide exchange factor; Septin-associated RhoGEF; SA-RhoGEF; p114RhoGEF; p114-Rho-GEF; Rho guanine nucleotide exchange factor 18; P114-RhoGEF |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6ZSZ5 Human Rho guanine nucleotide exchange factor 18 |
|---|---|
| Background | Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 152 kDa |
Images (1) Click the Picture to Zoom In
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ARG63434 anti-P114-RHO-GEF / ARHGEF18 antibody WB image
Western blot: 35 µg of Daudi (A) and Caco-2 (B) cell lysates (in RIPA buffer) stained with ARG63434 anti-P114-RHO-GEF / ARHGEF18 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
