ARG63132
anti-ORC3L antibody
anti-ORC3L antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ORC3L |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_862820.1 and NP_036513.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ORC3L |
| Antigen Species | Human |
| Immunogen | QKTDHVARLTWGGC |
| Conjugation | Un-conjugated |
| Alternate Names | LAT; Origin recognition complex subunit Latheo; ORC3L; LATHEO; Origin recognition complex subunit 3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UBD5 Human Origin recognition complex subunit 3 |
|---|---|
| Background | The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 82 kDa |
Images (2) Click the Picture to Zoom In
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ARG63132 anti-ORC3L antibody WB image
Western Blot: HeLa lysate (RIPA buffer, 1.4E5 cells per lane) stained with ARG63132 anti-ORC3L antibody at 2 µg/ml dilution.
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ARG63132 anti-ORC3L antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63132 anti-ORC3L antibody at 3.75 µg/ml dilution followed by AP-staining.
