ARG66154
anti-OPN / Osteopontin antibody
anti-OPN / Osteopontin antibody for ELISA and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes OPN / Osteopontin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | OPN / Osteopontin |
| Antigen Species | Human |
| Immunogen | HEK293 cells derived recombinant Human Osteopontin. (IPVKQADSGS SEEKQLYNKY PDAVATWLNP DPSQKQNLLA PQNAVSSEET NDFKQETLPS KSNESHDHMD DMDDEDDDDH VDSQDSIDSN DSDDVDDTDD SHQSDESHHS DESDELVTDF PTDLPATEVF TPVVPTVDTY DGRGDSVVYG LRSKSKKFRR PDIQYPDATD EDITSHMESE ELNGAYKAIP VAQDLNAPSD WDSRGKDSYE TSQLDDQSAE THSHKQSRLY KRKANDESNE HSDVIDSQEL SKVSREFHSH EFHSHEDMLV VDPKSKEEDK HLKFRISHEL DSASSEVN) |
| Conjugation | Un-conjugated |
| Alternate Names | BSPI; ETA-1; Uropontin; Osteopontin; Nephropontin; SPP-1; Bone sialoprotein 1; BNSP; Urinary stone protein; OPN; Secreted phosphoprotein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SPP1 |
| Gene Full Name | secreted phosphoprotein 1 |
| Background | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: OPN antibodies; OPN ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: The role of HDGF in tumor angiogenesis |
| Calculated MW | 35 kDa |
| PTM | Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. N- and O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62. |
Images (1) Click the Picture to Zoom In
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ARG66154 anti-OPN / Osteopontin antibody standard curve image
Sandwich ELISA: ARG66154 anti-OPN / Osteopontin antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66155 anti-OPN / Osteopontin antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
