ARG56066
anti-Neurofilament NF-L antibody [NR-4]
anti-Neurofilament NF-L antibody [NR-4] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Rat
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody
Overview
| Product Description | Mouse Monoclonal antibody [NR-4] recognizes Neurofilament NF-L |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | FACS, ICC/IF, IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NR-4 |
| Isotype | IgG1 |
| Target Name | Neurofilament NF-L |
| Antigen Species | Pig |
| Immunogen | Crude neurofilament preparation from Pig spinal cord. |
| Conjugation | Un-conjugated |
| Alternate Names | Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NEFL |
| Gene Full Name | neurofilament, light polypeptide |
| Background | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
| Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Highlight | Related products: Neurofilament NF L antibodies; Neurofilament NF L Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Neuronal Development Marker |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody |
| Calculated MW | 62 kDa |
| PTM | O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1. |
Images (2) Click the Picture to Zoom In
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ARG56066 anti-Neurofilament NF-L antibody [NR-4] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human cerebellum stained with ARG56066 anti-Neurofilament NF-L antibody [NR-4].
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ARG56066 anti-Neurofilament NF-L antibody [NR-4] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Rat cerebellum stained with ARG56066 anti-Neurofilament NF-L antibody [NR-4].
