ARG67021
anti-Nephrin antibody
anti-Nephrin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Nephrin |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Nephrin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-term region of human nephrin. |
| Conjugation | Un-conjugated |
| Alternate Names | CNF; NPHN; Renal glomerulus-specific cell adhesion receptor; nephrin; Nephrin |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Human Brain, HEK293, PC12 | ||||||
| Observed Size | ~135 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NPHS1 |
| Gene Full Name | nephrosis 1, congenital, Finnish type (nephrin) |
| Background | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
| Function | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). [UniProt] |
| Cellular Localization | Membrane |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 135 kDa |
| PTM | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. |
Images (2) Click the Picture to Zoom In
-
ARG67021 anti-Nephrin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG67021 anti-Nephrin antibody.
-
ARG67021 anti-Nephrin antibody WB image
Western blot: 1) HEK293, and 2) PC12 cell lysates stained with ARG67021 anti-Nephrin antibody.
