ARG66864
anti-NSE / Neuron Specific Enolase antibody
anti-NSE / Neuron Specific Enolase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NSE / Neuron Specific Enolase |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NSE / Neuron Specific Enolase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 371-420 of Human NSE / Neuron Specific Enolase. |
| Conjugation | Un-conjugated |
| Alternate Names | Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC 4.2.1.11 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HepG2 | ||||||||
| Observed Size | ~ 48 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ENO2 |
| Gene Full Name | enolase 2 (gamma, neuronal) |
| Background | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008] |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. [UniProt] |
| Calculated MW | 47 kDa |
Images (4) Click the Picture to Zoom In
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ARG66864 anti-NSE / Neuron Specific Enolase antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG66864 anti-NSE / Neuron Specific Enolase antibody (green) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG66864 anti-NSE / Neuron Specific Enolase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66864 anti-NSE / Neuron Specific Enolase antibody. The picture on the right is blocked with the synthetic peptide.
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ARG66864 anti-NSE / Neuron Specific Enolase antibody WB image
Western blot: HepG2 cell lysate stained with ARG66864 anti-NSE / Neuron Specific Enolase antibody at 1:2000 dilution.
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ARG66864 anti-NSE / Neuron Specific Enolase antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG66864 anti-NSE / Neuron Specific Enolase antibody. The picture on the right is blocked with the synthetic peptide.
