Overview

Product Description	Rabbit Polyclonal antibody recognizes NDUFA2
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	NDUFA2
Antigen Species	Human
Immunogen	Recombinant protein of Human NDUFA2
Conjugation	Un-conjugated
Alternate Names	CI-B8; Complex I-B8; CD14; NADH-ubiquinone oxidoreductase B8 subunit; CIB8; B8; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	Mouse kidney

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 17991 Mouse NDUFA2 GeneID: 4695 Human NDUFA2 Swiss-port # O43678 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Swiss-port # Q9CQ75 Mouse NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
Gene Symbol	NDUFA2
Gene Full Name	NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa
Background	The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
Function	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt]
Calculated MW	11 kDa

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