ARG42566

anti-NAA20 / NAT5 antibody

anti-NAA20 / NAT5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NAA20 / NAT5
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NAA20 / NAT5
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 49-178 of Human NAA20 / NAT5 (NP_057184.1).
Conjugation Un-conjugated
Alternate Names NatB complex subunit NAT5; N-terminal acetyltransferase B complex catalytic subunit NAA20; Methionine N-acetyltransferase; NAT3; NAT5P; dJ1002M8.1; NAT5; EC 2.3.1.88; NAT3P; N-acetyltransferase 5; N-terminal acetyltransferase B complex catalytic subunit NAT5; N-alpha-acetyltransferase 20; NatB catalytic subunit

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
IP1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse kidney
Observed Size ~ 17 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51126 Human NAA20

GeneID: 67877 Mouse NAA20

Swiss-port # P61599 Human N-alpha-acetyltransferase 20

Swiss-port # P61600 Mouse N-alpha-acetyltransferase 20

Gene Symbol NAA20
Gene Full Name N(alpha)-acetyltransferase 20, NatB catalytic subunit
Background NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM, Apr 2009]
Function Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration. [UniProt]
Cellular Localization Cytoplasm. Nucleus. [UniProt]
Calculated MW 20 kDa

Images (3) Click the Picture to Zoom In

  • ARG42566 anti-NAA20 / NAT5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human oophoroma tissue stained with ARG42566 anti-NAA20 / NAT5 antibody at 1:100 dilution.

  • ARG42566 anti-NAA20 / NAT5 antibody WB image

    Western blot: 25 µg of Mouse kidney lysate stained with ARG42566 anti-NAA20 / NAT5 antibody at 1:1000 dilution.

  • ARG42566 anti-NAA20 / NAT5 antibody IP image

    Immunoprecipitation: 200 µg extracts of MCF7 cells were immunoprecipitated and stained with ARG42566 anti-NAA20 / NAT5 antibody at 1:1000 dilution.