ARG42566
anti-NAA20 / NAT5 antibody
anti-NAA20 / NAT5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NAA20 / NAT5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NAA20 / NAT5 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 49-178 of Human NAA20 / NAT5 (NP_057184.1). |
| Conjugation | Un-conjugated |
| Alternate Names | NatB complex subunit NAT5; N-terminal acetyltransferase B complex catalytic subunit NAA20; Methionine N-acetyltransferase; NAT3; NAT5P; dJ1002M8.1; NAT5; EC 2.3.1.88; NAT3P; N-acetyltransferase 5; N-terminal acetyltransferase B complex catalytic subunit NAT5; N-alpha-acetyltransferase 20; NatB catalytic subunit |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse kidney | ||||||||
| Observed Size | ~ 17 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NAA20 |
| Gene Full Name | N(alpha)-acetyltransferase 20, NatB catalytic subunit |
| Background | NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM, Apr 2009] |
| Function | Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 20 kDa |
Images (3) Click the Picture to Zoom In
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ARG42566 anti-NAA20 / NAT5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oophoroma tissue stained with ARG42566 anti-NAA20 / NAT5 antibody at 1:100 dilution.
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ARG42566 anti-NAA20 / NAT5 antibody WB image
Western blot: 25 µg of Mouse kidney lysate stained with ARG42566 anti-NAA20 / NAT5 antibody at 1:1000 dilution.
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ARG42566 anti-NAA20 / NAT5 antibody IP image
Immunoprecipitation: 200 µg extracts of MCF7 cells were immunoprecipitated and stained with ARG42566 anti-NAA20 / NAT5 antibody at 1:1000 dilution.
