ARG41232
anti-Myotilin antibody
anti-Myotilin antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes Myotilin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Myotilin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human Myotilin. (C-DSQQHNSEHARLQ) (NP_006781.1; NP_001129412.1) |
| Conjugation | Un-conjugated |
| Alternate Names | TTOD; Titin immunoglobulin domain protein; LGMD1; TTID; 57 kDa cytoskeletal protein; Myofibrillar titin-like Ig domains protein; Myotilin; LGMD1A; MFM3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MYOT |
| Gene Full Name | myotilin |
| Background | This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.[provided by RefSeq, Oct 2008] |
| Function | Component of a complex of multiple actin cross-linking proteins. Involved in the control of myofibril assembly and stability at the Z lines in muscle cells. [UniProt] |
| Cellular Localization | Cell membrane, sarcolemma. Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line. Note=Sarcomeric, also localized to the sarcolemma (PubMed:10369880). Colocalizes with MYOZ1 at the Z-lines in skeletal muscle (PubMed:16076904). [UniProt] |
| Calculated MW | 55 kDa |
Images (1) Click the Picture to Zoom In
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ARG41232 anti-Myotilin antibody WB image
Western blot: 35 µg of Human skeletal muscle lysate (in RIPA buffer) stained with ARG41232 anti-Myotilin antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
