ARG59007
anti-Methylmalonyl-CoA mutase antibody
anti-Methylmalonyl-CoA mutase antibody for ICC/IF,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Methylmalonyl-CoA mutase |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Methylmalonyl-CoA mutase |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 492-526 of Human Methylmalonyl-CoA mutase. |
| Conjugation | Un-conjugated |
| Alternate Names | Methylmalonyl-CoA isomerase; MCM; EC 5.4.99.2; Methylmalonyl-CoA mutase, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P16332 Mouse Methylmalonyl-CoA mutase, mitochondrial Swiss-port # P22033 Human Methylmalonyl-CoA mutase, mitochondrial |
|---|---|
| Gene Symbol | MUT |
| Gene Full Name | methylmalonyl CoA mutase |
| Background | This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008] |
| Function | Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species. [UniProt] |
| Cellular Localization | Mitochondrion matrix. [UniProt] |
| Calculated MW | 83 kDa |
Images (2) Click the Picture to Zoom In
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ARG59007 anti-Methylmalonyl-CoA mutase antibody ICC/IF image
Immunofluorescence: 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells stained with ARG59007 anti-Methylmalonyl-CoA mutase antibody (green) at 1:25 dilution. Nuclear counter stain is DAPI (blue).
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ARG59007 anti-Methylmalonyl-CoA mutase antibody WB image
Western blot: 20 µg of K562 cell lysate stained with ARG59007 anti-Methylmalonyl-CoA mutase antibody at 1:2000 dilution.
