ARG43105
anti-MTDH / AEG1 antibody
anti-MTDH / AEG1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes MTDH / AEG1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | MTDH / AEG1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant fragment of Human MTDH / AEG1. |
| Conjugation | Un-conjugated |
| Alternate Names | 3D3/LYRIC; AEG-1; Metastasis adhesion protein; 3D3; LYRIC/3D3; AEG1; Protein LYRIC; Lysine-rich CEACAM1 co-isolated protein; LYRIC; Metadherin; Astrocyte elevated gene-1 protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | MCF7 | ||||||||
| Observed Size | ~ 73 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Unpurified |
| Buffer | Ascitic fluid and 0.03% Sodium azide. |
| Preservative | 0.03% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MTDH |
| Gene Full Name | metadherin |
| Function | Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. [UniProt] |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Cell junction, tight junction. Nucleus, nucleolus. Cytoplasm, perinuclear region. Note=In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus. [UniProt] |
| Calculated MW | 64 kDa |
Images (3) Click the Picture to Zoom In
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ARG43105 anti-MTDH / AEG1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG43105 anti-MTDH / AEG1 antibody.
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ARG43105 anti-MTDH / AEG1 antibody WB image
Western blot: MCF7 cell lysate stained with ARG43105 anti-MTDH / AEG1 antibody at 1:5000 dilution.
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ARG43105 anti-MTDH / AEG1 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG43105 anti-MTDH / AEG1 antibody (right histogram) and negative control (left histogram).
