ARG63477

anti-MRPL3 antibody

anti-MRPL3 antibody for Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes MRPL3
Tested Reactivity Hu
Predict Reactivity Cow, Pig
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name MRPL3
Antigen Species Human
Immunogen NVCQPGAPSITFA
Conjugation Un-conjugated
Alternate Names RPML3; L3mt; MRL3; MRP-L3; COXPD9; 39S ribosomal protein L3, mitochondrial

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11222 Human MRPL3

Swiss-port # P09001 Human 39S ribosomal protein L3, mitochondrial

Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody
Calculated MW 39 kDa

Images (1) Click the Picture to Zoom In

  • ARG63477 anti-MRPL3 antibody WB image

    Western Blot: HepG2 cell lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63477 anti-MRPL3 antibody at 1 µg/ml dilution.