ARG44193

anti-MIS12 antibody

anti-MIS12 antibody for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes MIS12
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MIS12
Antigen Species Human
Immunogen Recombinant protein of Human MIS12
Conjugation Un-conjugated
Protein Full Name Protein MIS12 homolog
Alternate Names MIS12; MIS12 Kinetochore Complex Component; KNTC2AP; MTW1; Protein MIS12 Homolog; MGC2488; HMIS12; MIS12, MIND Kinetochore Complex Component, Homolog (S. Pombe); MIS12, MIND Kinetochore Complex Component, Homolog (Yeast); MIS12, MIND Kinetochore Complex Component, Homolog; Homolog Of Yeast Mis12; 2510025F08Rik; MIS12 Homolog; HMis12

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
WB0.1-0.25 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 79003 Human MIS12

Swiss-port # Q9H081 Human Protein MIS12 homolog

Gene Symbol MIS12
Gene Full Name Mis12 Kinetochore Complex Component
Background Involved in attachment of mitotic spindle microtubules to kinetochore and kinetochore assembly. Located in kinetochore and nucleus. Part of MIS12/MIND type complex.
Function Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis.
Cellular Localization Centromere, Chromosome, Kinetochore
Calculated MW 24 kDa

Images (3) Click the Picture to Zoom In

  • ARG44193 anti-MIS12 antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG44193 anti-MIS12 antibody at 5 μg/mL dilution.

  • ARG44193 anti-MIS12 antibody WB image

    Western blot: HeLa, 293T, HepG2 and Jurkat stained with ARG44193 anti-MIS12 antibody at 0.5 μg/mL dilution.

  • ARG44193 anti-MIS12 antibody FACS image

    Flow Cytometry: JK stained with ARG44193 anti-MIS12 antibody at 1 μg/1x10^6 cells dilution.