ARG43405
anti-METTL16 antibody
anti-METTL16 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes METTL16 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | METTL16 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 263-562 of Human METTL16 (NP_076991.3). |
| Conjugation | Un-conjugated |
| Alternate Names | Methyltransferase 10 domain-containing protein; METT10D; Methyltransferase-like protein 16; EC 2.1.1.- |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | A549 | ||||||||||
| Observed Size | ~ 74 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | METTL16 |
| Gene Full Name | methyltransferase like 16 |
| Function | RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197299, PubMed:30197297). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:28525753). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200). Also able to bind various lncRNAs (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 64 kDa |
Images (4) Click the Picture to Zoom In
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ARG43405 anti-METTL16 antibody ICC/IF image
Immunofluorescence: C6 cells stained with ARG43405 anti-METTL16 antibody at 1:100 dilution.
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ARG43405 anti-METTL16 antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG43405 anti-METTL16 antibody at 1:1000 dilution.
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ARG43405 anti-METTL16 antibody IP image
Immunoprecipitation: 300 µg extracts of LNCaP cells were immunoprecipitated and stained with ARG43405 anti-METTL16 antibody at 1:1000 dilution.
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ARG43405 anti-METTL16 antibody WB image
Western blot: 25 µg of Rat testis lysate stained with ARG43405 anti-METTL16 antibody at 1:1000 dilution.
