ARG59176
anti-MED14 antibody
anti-MED14 antibody for Flow cytometry,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MED14 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MED14 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 586-615 of Human MED14. |
| Conjugation | Un-conjugated |
| Alternate Names | CSRP; Transcriptional coactivator CRSP150; TRAP170; RGR1; Mediator complex subunit 14; Thyroid hormone receptor-associated protein complex 170 kDa component; CRSP complex subunit 2; CXorf4; Vitamin D3 receptor-interacting protein complex 150 kDa component; DRIP150; CRSP150; ARC150; CRSP2; EXLM1; RGR1 homolog; Trap170; hRGR1; Activator-recruited cofactor 150 kDa component; Cofactor required for Sp1 transcriptional activation subunit 2; Mediator of RNA polymerase II transcription subunit 14 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | DU145 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # A2ABV5 Mouse Mediator of RNA polymerase II transcription subunit 14 Swiss-port # O60244 Human Mediator of RNA polymerase II transcription subunit 14 |
|---|---|
| Gene Symbol | MED14 |
| Gene Full Name | mediator complex subunit 14 |
| Background | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008] |
| Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 161 kDa |
Images (2) Click the Picture to Zoom In
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ARG59176 anti-MED14 antibody WB image
Western blot: 20 µg of DU145 cell lysate stained with ARG59176 anti-MED14 antibody at 1:4000 dilution.
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ARG59176 anti-MED14 antibody FACS image
Flow Cytometry: A549 cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by ARG59176 anti-MED14 antibody (right histogram) at 1:25 for 60 min at 37°C, followed by DyLight®488 labelled secondary antibody. Isotype control antibody (left histogram) was rabbit IgG1 (1 µg/10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
